Hitachi Announces the Launch of the New EX2000-7 Excavator for

  • Structure of a helicase–helicase loader complex reveals

    Sep 19, 2013 · During the assembly of the bacterial loader-dependent primosome, helicase loader proteins bind to the hexameric helicase ring, deliver it onto the …

    Learn More
  • The Bacterial DnaC Helicase Loader Is a DnaB Ring …

    structure of the intact E. coli DnaB,DnaC helicase/ loader complex. The 480 kDa dodecamer forms a three-tieredassembly,inwhichDnaCadoptsaspiral configuration that remodels N-terminal scaffolding and C-terminal motor regions of DnaB to produce a clear break in the helicase ring. Surprisingly, DnaC's AAA+ fold is dispensable for ring remodeling

    Learn More
  • Cureus | Structure of a helicase–helicase loader complex

    Sign In; Create an Account "Never doubt that a small group of thoughtful, committed citizens can change the world. Indeed, it is the only thing that ever has."

    Learn More
  • The X-ray structure of the papillomavirus helicase in

    DNA replication of the papillomaviruses is specified by cooperative binding of two proteins to the ori site: the enhancer E2 and the viral initiator E1, a distant member of the AAA+ family of proteins. Formation of this prereplication complex is an essential

    Learn More
  • (PDF) Insights into the structure and assembly of the

    Insights into the structure and assembly of the Bacillus subtilis clamp-loader complex and its interaction with the replicative helicase 12 Pages Nucleic Acids Research, 2013

    Learn More
  • Structure of a helicase–helicase loader complex reveals

    Dec 01, 2012 · Structure determination. The complex of BstDnaB helicase, BsuDnaI loader and the HBD of BstDnaG primase was obtained by first purifying the DnaB–DnaI complex using gel filtration chromatography and then adding the HBD of DnaG (Fig. 1).This procedure is the same as previously reported15.Analysis of the proteins in these crystals using mass spectrometry and tryptic digestion …

    Learn More
  • Mechanisms in the loading of the replicative DNA helicase

    By using ATPγS, an ATP analogue, which can be only very slowly hydrolysed, we successfully captured a helicase loading intermediate. This complex, which contains all 14 pre-RC polypeptides, reveals for the first time how the eukaryotic helicase interacts with its loader. The structure identifies how ORC/Cdc6 and Cdt1/MCM2-7 interact with each

    Learn More
  • EM structure of a helicase-loader complex depicting a 6:2

    Request PDF | On Mar 1, 2016, Yen-Chen Lin and others published EM structure of a helicase-loader complex depicting a 6:2 binding sub-stoichiometry from Geobacillus kaustophilus HTA426 | Find

    Learn More
  • Crystal structure of the complex of the interaction

    Request PDF | Crystal structure of the complex of the interaction domains of E. coli DnaB helicase and DnaC helicase loader: Structural basis implying a distortion-accumulation mechanism for the

    Learn More
  • Structure of hexameric DnaB helicase and its complex with

    The complex between the DnaB helicase and the DnaG primase unwinds duplex DNA at the eubacterial replication fork and synthesizes the Okazaki RNA primers. The crystal structures of hexameric DnaB and its complex with the helicase binding domain (HBD) of DnaG reveal that within the hexamer the two domains of DnaB pack with strikingly different symmetries to form a distinct two-layered ring

    Learn More
  • PDF.MPro - Full Text Journal Article: Crystal structure of

    Crystal structure of the complex of the interaction domains of Escherichia coli DnaB helicase and DnaC helicase loader: structural basis implying a distortion-accumulation mechanism for the DnaB ring opening caused by DnaC binding. PMID: 31665315 (view PubMed database entry) DOI: 10.1093/jb/mvz087 (read at publisher's website )

    Learn More
  • Crystal structure of the complex of the interaction

    The bound helicase loader protein, EcDnaC N, EcDnaC or λP, and the linker helix of the adjacent EcDnaB subunit are also shown. EcDnaC N, EcDnaC C and λP are coloured red, magenta and orange, respectively. (A) Overall structure of the helicase loader–EcDnaB C –EcDnaB L ' complex.

    Learn More
  • EM structure of a helicase-loader complex depicting a 6:2

    Apr 22, 2016 · This nearly undistorted structure describes a state after the recruitment of primase, which might consequently induce a conformational change in the preformed helicase-loader complex. Meanwhile, helicase-loader complexes with sub-stoichiometries (6:1 and 6:2) were also documented in B. subtilis species,,, but their structural detail is

    Learn More
  • Structure of a helicase-helicase loader complex reveals

    Structure of a helicase-helicase loader complex reveals insights into the mechanism of bacterial primosome assembly Bin Liu, William K. Eliason, Thomas A. Steitz Hormel Institute

    Learn More
  • Structure of a helicase-helicase loader complex reveals

    Jan 01, 2013 · Structure determination. The complex of BstDnaB helicase, BsuDnaI loader and the HBD of BstDnaG primase was obtained by first purifying the DnaB–DnaI complex using gel filtration chromatography and then adding the HBD of DnaG (Fig. 1).This procedure is the same as previously reported15.Analysis of the proteins in these crystals using mass spectrometry and tryptic digestion …

    Learn More
  • EM structure of a helicase-loader complex depicting a 6:2

    EM structure of a helicase-loader complex depicting a 6:2 binding sub-stoichiometry from Geobacillus kaustophilus HTA426 Author: Lin, Yen-Chen, Naveen, Vankadari, Hsiao, Chwan-Deng Source: Biochemical and biophysical research communications 2016 v.473 pp. 243-248 ISSN: 0006-291X Subject

    Learn More
  • Structure of a helicase-helicase loader complex reveals

    Sep 01, 2013 · During the assembly of the bacterial loader-dependent primosome, helicase loader proteins bind to the hexameric helicase ring, deliver it onto the oriC DNA and then dissociate from the complex. Here, to provide a better understanding of this key process, we report the crystal structure of the ~570-kDa prepriming complex between the Bacillus subtilis loader protein and the Bacillus

    Learn More
  • The crystal structure of a replicative hexameric helicase

    Dec 15, 2008 · The GkDnaC–ssDNA complex structure reveals that three symmetrical basic grooves on the interior surface of the hexamer individually encircle ssDNA. The ssDNA-binding pockets in this structure are directed toward the N-terminal domain collar of the hexameric ring, thus orienting the ssDNA toward the DnaG primase to facilitate the synthesis of short RNA primers.

    Learn More
  • Crystal structure of the complex of the interaction

    Loading the bacterial replicative helicase DnaB onto DNA requires a specific loader protein, DnaC/DnaI, which creates the loading-competent state by opening the DnaB hexameric ring. To understand the molecular mechanism by which DnaC/DnaI opens the DnaB ring, we solved 3.1-Å co-crystal structure of …

    Learn More
  • Structure of a helicase–helicase loader complex reveals

    Sep 19, 2013 · Structure of a helicase–helicase loader complex reveals insights into the mechanism of bacterial primosome assembly Structure of a helicase–helicase loader complex reveals insights into the mechanism of bacterial primosome assembly. Access Restriction Open. Author: Liu, Bin ♦ Eliason, William K. ♦ Steitz, Thomas A.

    Learn More